This medium has a low nutrient content to prevent replication of organisms while maintaining viability. Sodium thioglycollate is added to impede oxidation. Disodium phosphate is incorporated in the medium as a buffering agent to prevent overgrowth of enteric pathogens with Escherichia coli and Enterobacter. The alkaline pH minimizes the destruction of bacteria due to acid formation.
1. If a stool specimen is received, place approximately one gram of fecal material into Cary Blair Transport Medium. If specimen is received
on a swab, place the swab in Cary Blair Transport Medium and cut off the protruding portion of the swab.
2. Secure the cap tightly and label the specimen.
3. Transport the specimen to the laboratory with minimal delay at room temperature unless recovery of Shigella is required, in which case
the specimen should be maintained at 2-8°C during shipment.
4. Mix the specimen thoroughly prior to inoculation of appropriate media.
All lot numbers of Cary Blair Transport Medium have been tested using the following quality control organisms and have been found to be
acceptable. Testing of control organisms should be performed in accordance with established laboratory quality control procedures. If
aberrant quality control results are noted, patient results should not be reported.